By Luke Sumpter


Researchers have discovered that introducing sucrose directly into the stem of flowering cannabis plants, a technique called PSIS, can significantly boost flower dry mass and total cannabinoid yield without disrupting chemotype or plant balance.

This simple, low-pressure method could become a valuable future tool for both commercial and home growers seeking to enhance production in a clean and biologically aligned manner. Discover everything you need to know about PSIS below.

Introducing the Rising Cannabis Market

The modern cannabis landscape is changing quickly. Genetics continue to advance, environments are becoming more controlled, and growers are becoming more informed than ever before. From hobby tents to licensed facilities, one theme remains consistent: everyone wants the healthiest plants possible, and everyone wants more consistent and reliable buds when harvest time arrives.

Indoor cannabis grow in full flowering stage using PSIS technique (stem sucrose injection) to increase bud dry weight and total cannabinoid yield

Innovations in cannabis cultivation sometimes come from complex tech or new breeding strategies. Other times, they come from surprisingly simple places. The study discussed here centres on one of the simplest inputs imaginable: plain table sugar (sucrose).

A recent study[1] conducted between the University of Ljubljana, Slovenia, and the Czech University of Life Science Prague, Czech Republic, explored a novel approach called PSIS, short for plant stem infusion of sucrose.

Instead of relying on standard soil feeding or foliar sprays, PSIS introduces a carefully controlled sucrose solution directly into the plant stem at a stable pressure during the flowering period. The idea is simple: adding extra sucrose provides the plant with more carbon at the precise moment when flowers need it most, directing more energy into buds rather than excess leaf growth.

Close-up of PSIS technique: sucrose solution being injected directly into the cannabis stem during flowering stage to boost bud weight and cannabinoid production

What makes this study so interesting is the non-disruptive nature of the intervention. PSIS improved cannabinoid yield and flower dry mass, but photosynthesis parameters barely changed. Instead of “forcing” a plant to work harder or pushing its metabolism into stress territory, the change seemed to help the plant make smarter decisions internally about where to place its energy, carbon, and structural biomass.

This subtle shift is what makes PSIS one of those rare innovations that is both scientifically novel and conceptually accessible to the general grower. Overall, this study uncovered a simple but effective input that could easily find a place amongst both home growers and commercial cultivators.

What Is Plant Stem Infusion of Sucrose (PSIS)?

PSIS is a targeted stem-based delivery method. In practical terms, a sterile line feeds a specific concentration of sucrose directly into the plant stem using controlled low pressure.

The carbon from the sucrose enters the internal transport pathways of plants directly, bypassing the natural bottlenecks and variables that come from soil/root-based nutrient delivery.

To appreciate why this is different, it helps to contrast this with methods most growers already know:

  • Foliar feeding: Has short windows of benefit and risks tissue burn or leaf stress if concentrations are too high.
  • Root feeding: Relies on soil chemistry, microbial communities, water distribution, root health, and transpiration. There are many benefits, but also many potential limitations.

By comparison, PSIS allows the movement of sucrose through the vascular system using controlled pressure. Because the study maintained relatively low pressure in the most successful treatments, the plants were not mechanically overwhelmed.

Instead, they received additional carbon with minimal disruption to their normal metabolism. This direct infusion provided a steady and predictable way to boost yield without the risk of destabilising the entire system.

It is also important to remember that sucrose is not just an energy source. In plants, it also acts as a regulatory signal. When it appears in specific locations and ratios within the plant, it influences developmental outcomes.

It can “tell” tissues where to act like sinks (pulling resources in) versus where to act like sources for other parts of the plant. Flowers are strong natural sinks, and during flowering, introducing more sucrose in a controlled way appears to reinforce that sink behaviour.

This is likely why the study showed a substantial increase in flower dry mass and cannabinoid yield under the right pressure conditions, even though classical measures of photosynthetic output changed only slightly.

The Test: Method & Design

To explore the potential of PSIS, researchers conducted controlled experiments on 72 plants of Charlotte’s Angel, a well-known high-CBD, low-THC chemotype. This choice of plant material also helps isolate the changes in total cannabinoid yield without generating major concerns about unexpected increases in THC content, because the baseline genetic expression is already strongly tilted toward CBD dominance.

The study tested multiple sucrose concentrations (0%, 7.5%, 15%, and 30%) and combined each with three different pressure levels: 0.5 bar, 1 bar, and 2 bar. Infusions were applied during early flowering, and the researchers assessed numerous parameters, including plant height, total structural biomass, and physiological measurements, such as photosynthesis.

Charlotte’s Angel PSIS test design 72 high CBD low THC plants testing sucrose concentrations 0% 7.5% 15% 30% and pressure levels 0.5 bar 1 bar 2 bar

The Results: How PSIS Enhances Cannabis Yields

The most important discovery from the work was how clearly gentle pressure stood out. At 0.5 bar, PSIS paired with 15–30% sucrose produced the strongest positive outcomes: flower dry mass increased by up to 31% and cannabinoid yield rose by as much as 34% per plant.

The plants receiving PSIS at 0.5 bar were taller, with significantly greater flower dry mass and stem mass. Meanwhile, as pressure increased, results reversed. At 1 bar, plants exhibited spikes in respiration, indicating that they were burning additional carbon instead of storing it in structural tissues or cannabinoids.

At 2 bar pressure, some treatment groups even underperformed against controls in terms of cannabinoid yield.

Graph showing effect of sucrose concentration in PSIS technique up to 31% increase in flower dry mass and 34% higher total cannabinoid yield at 15% to 30% sucrose

Another important detail was that the chemotype didn’t change. Although flower dry mass increased and total cannabinoid yield rose with PSIS, CBD remained the dominant cannabinoid, and THC levels remained very low, similar to those of the control plants.

This indicates that the improvement resulted from the plant investing more energy in flower production, rather than from altering the balance of cannabinoids themselves. For growers working with regulated or specific cannabinoid profiles, this stability is a significant advantage in maintaining levels below certain thresholds.

What Can PSIS Do For Cannabis Growers?

The findings from this study point toward a promising new direction in cannabis cultivation. PSIS won’t replace fertilisers, soil biology, genetics, or the fundamentals growers already rely on. Instead, it will likely serve as a subtle, well-timed support method that adds focused carbon exactly when flowers need it most, boosting yield and cannabinoid content where required.

For commercial operations, this approach could eventually become a scalable way to nudge yields higher without having to overhaul entire feeding strategies. And because the mechanism is so simple, it may be easier to trial, measure, and validate than more complicated alternatives.

For smaller tents and home growers, PSIS is a technique that could find a natural place in the future, once further testing and refinement are completed. It blends scientific grounding with a concept that’s intuitively easy to understand: carbon matters when buds are building fast.

The bigger picture? PSIS shows that gentle, well-timed carbon support can steer energy toward the parts we actually harvest, without disrupting plant balance or changing chemotypes.

Disclaimer:
This content is for educational purposes only. The information provided is derived from research gathered from external sources.

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Privacy Policy - Royal Queen Seeds

To ensure a safe online environment and guarantee adequate data protection, we strictly comply with all legal requirements. In this privacy statement, we provide information about how and for what purpose data is collected, safety measures, storage periods and contact details.


COMPANY NAME: SNORKEL SPAIN, SL (hereinafter ROYAL QUEEN SEEDS)

C/ Vilar d'Abdelà, 5 (nave 1) CP: 08170 de Montornès del Vallès

+34 937 379 846

[email protected]


The present Privacy Policy sets out the terms on which we will treat personal data at ROYAL QUEEN SEEDS; this includes any personal data collected through our website https://www.royalqueenseeds.com/ as well as any other data we process in the course of our business activities.

ROYAL QUEEN SEEDS collects the following personal data for the purposes listed below:

SECTION 1 – PERSONAL INFORMATION WE COLLECT

1.1 Account purchases

Account purchases can only be made if you are in possession of a personal account. When you create an account or purchase something from our shop, as part of the buying and selling process we collect the following personal information that you provide to us:

This information is required for delivery. In addition, when you browse our shop, we automatically receive the Internet Protocol (IP) address of your computer. Based on this information, we can optimise your online experience and at the same time protect our online environment.

Purpose of data collection

We collect and store account-related data for the following purposes:

(a) to carry out obligations arising from any contracts between you and us, and to provide you with information, products and services that you may request from us;

(b) to set up, manage and communicate with you about your account and your orders;

(c) to conduct market research and analysis;

(d) to confirm your age and identity, and to detect and prevent fraud.

1.2 Newsletters

With your explicit permission, we may send you newsletters about our shop, new products and other updates. We send newsletters based on your explicit consent. In the event that you purchase a product, and in accordance with current regulations, we may send you commercial communications in accordance with the legitimate interest of our company, always about products or services similar to those you have purchased or contracted. In any case, you may exercise your right of opposition through the channels announced in this Privacy Policy. The following information is collected in relation to the newsletter:

We do not need to know the sex of the person in order to send the newsletter (data minimisation: by law we must ask for data that is strictly necessary to provide the service, and in this case knowing the sex is not necessary to send the newsletter).

Purpose of data collection

The data collected is used to:

(a) personalise our emails, including your name and gender;

(b) provide gender-specific content.

You can withdraw your consent at any time by using the link provided in the newsletter or the contact information provided in section 2.

1.3 Customer service and contact form

In order to provide appropriate support, our customer service employees have access to information related to the account. Consequently, their support will be highly effective and friendly. The data provided in our contact form is used by our CRM provider, SuperOffice. We will only use your details to respond to your message.

SECTION 2 – LEGITIMATE INTEREST

If you have purchased any of our products, please note that we may process your personal data for promotional purposes, based on Royal Queen Seeds' legitimate interest only to offer you products or services from our company and about products or services similar to those you have purchased. You may exercise your right to opt out of future messages by the means set out in this Privacy Policy or through any notification you receive.

2.1 How do you withdraw consent?

If you change your mind, you can withdraw your consent for us to contact you for the purpose of collecting, using, or disclosing your data at any time by reaching out to us at: [email protected].

SECTION 3 – DISCLOSURE

We may disclose your personal information if we are required to do so by law or if you breach our Terms of Service.

SECTION 4 – HOW LONG DO WE KEEP YOUR DATA?

At Royal Queen Seeds, we will not retain your data for longer than is necessary for the purposes described in this Policy. Different retention periods apply for different types of data; however, the longest period we will normally hold any personal data is 10 years.

4.1 Account information

Data relating to the account remains relevant for as long as the consumer is in possession of an account. Therefore, the data remains documented for as long as the account exists. When our customers delete an account, the associated data will be deleted within a reasonable period of time. Requests regarding the inspection or correction of stored personal data or the deletion of an account can be sent to [email protected].

4.2 Newsletters

In the event that you give us your consent to inform you about our products or services, we will keep your data until you express your wish not to receive any further communications from us. However, we regularly (every month) carry out a relevance check. Registered customers (and their personal information) will be deleted whenever customers do not reply to our request. In addition, our newsletter mailing has an opt-out feature. Consumers can withdraw their consent by using this opt-out feature.

SECTION 5 – COOKIES

Cookies are small information files that notify your computer of previous interactions with our website. These cookies are stored on your hard drive, not on our website. Essentially, when you use our website, your computer displays its cookies to us, informing our site that you have visited before. This allows our website to function more quickly and remember aspects related to your previous visits (such as your username), making your experience more convenient. At Royal Queen Seeds, we use two types of cookies: functional and analytical.

5.1 Functional Cookies

Functional cookies are used to enhance your online experience. Among other things, these cookies track what is added to your shopping cart. The use of these cookies does not require prior authorization.

5.2 Analytical Cookies

Analytical cookies are used for research and market analysis. The data collected with these analytical cookies is anonymous, making it unusable for third parties. The use of these cookies does not require prior authorization.

SECTION 5 – THIRD-PARTY SERVICES

Third-party services are required to conduct transactions and provide our services. In general, the third-party providers we use will only collect, use and disclose your information to the extent necessary to enable them to perform the services they provide to us.

However, certain third-party service providers, such as payment gateways and other payment transaction processors, have their own privacy policies regarding the information we must provide to them for your transactions.

We encourage you to read the privacy policies of these providers so that you can understand how these providers will handle your personal information.

In particular, certain suppliers may be located or have facilities located in a different jurisdiction than yours or ours. Therefore, if you choose to proceed with a transaction involving the services of a third party, your information may be subject to the laws of the jurisdiction in which that service provider or its facilities are located.

Once you leave our website or are redirected to a third-party website or application, you are no longer governed by this Privacy Policy or the Terms of Service of our website.

Web analytics service (anonymous data)

On this website we have integrated an element of a web analytics service (with anonymisation functionality). Web analytics can be defined as the gathering, processing and analysis of data about the behaviour of visitors to websites. An analytics service collects, among other things, data about which website a person came from (the so-called referrer), which sub-pages they visited or how often and for how long they visited a sub-page. Web analytics is mainly used for website optimisation and for a cost–benefit analysis of internet advertising.

Courier service

To complete deliveries we use a courier service. This courier service carries out the delivery between our company and the consumer's home. To complete these logistics, the company requires access to the consumer's name and address information.

Mailing service

Royal Queen Seeds uses a third-party mail service provider to send its newsletter. This provider has access to limited account information related to opt-in consent (e.g. email address).

Marketing services

Royal Queen Seeds has the support of a company that specialises in marketing and communication activities. Their access to personal information is very limited and mostly anonymous.

Payment services

At Royal Queen Seeds we use external payment services to handle our transactions (e.g. credit card payments).

SECTION 6 – SECURITY

To protect your personal information, we take reasonable precautions and follow industry standard best practices to ensure that it is not inappropriately lost, misused, accessed, disclosed, altered or destroyed.

If you provide us with your credit card information, the information is encrypted using secure socket layer technology (SSL) and stored using AES-256 encryption. Although no method of transmission over the internet or electronic storage is 100% secure, we follow all PCI-DSS requirements and implement additional industry standards that are commonly accepted. Information related to the account is protected with a hashing method. This method transforms the information into a generated hash. As a result, confidential information is protected and invisible, even to us. In addition, our databases are exceptionally protected against unauthorised access. For example, access to the database is only possible and permitted via approved IP addresses (e.g. from Royal Queen Seeds headquarters). Other attempts and addresses are rejected at all times.

Furthermore, data is anonymised as much as possible, so it cannot be directly linked to a specific consumer. With this data, however, we may be able to carry out market research and analysis. In addition, the third parties concerned (e.g. mailing service) are examined prior to our collaboration, comply with the GDPR from the EU and receive a processing agreement. Within Royal Queen Seeds, employees are assigned different access permissions. Specific permission provides access only to information that is strictly required to perform a task. Digital security measures are subject to change and must meet high requirements to ensure the safety of online customers. That is why, at Royal Queen Seeds, we appoint a security officer. Regular verification and improvement of security measures (where necessary) are part of the role.

SECTION 7 – CHANGES TO THIS PRIVACY POLICY

We reserve the right to modify this Privacy Policy at any time, so please check back regularly. Changes and clarifications will be effective immediately upon being published on the Website. If we make substantial changes to this policy, we will notify you here that it has been updated so you will know what information we collect, how we use it and under what circumstances, if any, we use and/or disclose it.

SECTION 8 – YOU HAVE THE RIGHT TO: