By Luke Sumpter


Despite the work we’ve put into developing our line of F1 hybrids at RQS, we know that genetics are only half of the equation. Substrate choice, irrigation, lighting, and nutrition all strongly influence how each plant expresses its traits, ultimately shaping growth and final yields.

To help you get the best possible results, we’ve carried out rigorous cultivation trials together with Vertify to develop clear, evidence-based standard operating procedures that you can apply directly in your grow. These trials reveal which environmental factors allow our F1 hybrids to reach their full potential: water type, growing media, nutrient levels, planting density, and organic conditions.

Key Takeaways

  • F1 hybrid trials performance: Gaia F1 demonstrated remarkable uniformity and compact growth across both rockwool and coco, confirming that F1 seeds are stable under well-managed conditions.
  • Substrate trade-offs: Rockwool provided predictable and consistent yields, ideal for reliable production, while coco offered slightly higher potential yields but with greater variability.
  • Treatment impacts: Rainwater and low WC treatments performed poorly, highlighting the importance of controlled irrigation and nutrient regimes for commercial cultivation.
  • Optimising yields: Harvest indices ranged from 54% to 69%, generally higher in rockwool. Future trials with denser planting (up to 8–9 plants/m²) could push yields toward 1.5 kg/m², particularly under optimised conditions at CRIC Labs.

Vertify Trials: A Trusted Hub for Plant Innovation

To get the best data, we partnered with Vertify, one of Europe’s top applied horticultural research centres. Founded in 1986, Vertify now runs advanced greenhouses and trial fields at Zwaagdijk and the World Horti Center. With decades of experience and 25+ projects a year, Vertify is the ideal partner for rigorous cannabis testing.

Controlled trial of Gaia F1 hybrid cannabis testing substrates, irrigation, and growth performance under identical conditions.

Introducing Gaia F1

Gaia F1 is a cross between New Breed Auto and Black Domina, which stands out for its exceptional performance. This hybrid is the result of a breeding program that focuses on genetic stability, uniformity, and enhanced pest tolerance.

With high THC levels and relaxing terpenes, Gaia F1 delivers a calming effect with sweet, citrusy flavours and hints of mint.

Gaia F1
23_genetic background_1 New Breed Auto x Black Domina x Sin Trabajo
25_flowering time_1 40 - 45 days
29_THC_1 THC: Very high
22_Effect_1 Physically Relaxing, Sleepy, Stoned
110_harvest 65 - 70 days after germination

Buy Gaia F1

She was designed to grow compact, fast, and predictably, reaching 50–70cm in height and finishing in just 65–70 days from germination, while still producing dense, resinous buds.

Close up of the hybrid Gaia F1

Trial Design and Methodology: Inside the Gaia F1 Hybrid Trial

The trial took place between December 2024 and February 2025 and was designed to analyse how water quality, nutrient intensity, and application methods interact with F1 cannabis stability across 384 plants.

Rockwool and coco were grown under near-identical conditions of light (800 µmol/m²/s with an 18-hour photoperiod), temperature, and humidity to isolate the effects of substrate and treatments. All plants were spaced at six plants per square metre, simulating both home and intensive commercial setups.

Researchers recorded irrigation frequency and plant height on a weekly basis. At harvest, inflorescences were weighed fresh and dry, and yields were expressed in grams per plant (g/plant) and kilograms per square metre (kg/m²).

Infographic of Gaia F1 cannabis trial featuring 384 plants under 800 μmol/m²/s LED light, 18-hour photoperiod, in rockwool and coco substrates, near-identical conditions, 6 plants per m².

Control

In cultivation trials, a control treatment is a group of plants managed under standard or reference conditions, serving as the benchmark to evaluate the effects of all other treatments.

In this case, the control group received a balanced NPK solution with moderate electrical conductivity (EC) and irrigation at standard intervals. By establishing this condition as the starting point, Vertify was able to assess how the other treatments influenced yield, F1 hybrid stability, and overall plant vigour.

Treatment 1: Enhanced Nutrients

The enhanced nutrients group in the cannabis substrate trials prompted Gaia F1 to adopt more intensive feeding. Interestingly, previous research has shown that higher nitrogen and potassium during flowering can increase biomass, but sometimes dilute cannabinoid concentrations[1]. By establishing this group, Vertify aimed to find the optimal balance between flower density and reduced potency.

Treatment 2: Reduced Nutrients

Reducing total nitrogen, phosphorus, and potassium (NPK) at all stages simulated a low-input regime. Studies on both hemp and cannabis suggest that moderate nutrient stress, although counterintuitive, can trigger secondary metabolite production, potentially boosting levels of terpenes including myrcene and caryophyllene[1]. However, prolonged deficiency may also reduce both yields and potency.

Treatment 3: Rainwater

In this treatment, researchers replaced mineralised irrigation water with collected rainwater, which has a very low EC. In horticulture, rainwater’s lack of dissolved salts allows precise nutrient dosing but also increases the risk of pH instability. Rainwater may reduce salt stress, but could lead to micronutrient deficiencies if not supplemented correctly. Data from this group is significant to outdoor growers of F1 hybrids, especially those who are more self-sufficient and make an effort to harvest rainwater.

Treatment 4: Low WC (Water Content)

Restricted irrigation frequency created a controlled drought-like environment. Previous studies have shown that water stress can modulate the levels of cannabinoids at various growth stages, including THC, CBD, and CBN[2]. This treatment tested Gaia F1’s resilience, root zone efficiency, and overall response to water stress.

Controlled drought setup testing Gaia F1’s response to water stress and cannabinoid changes.

Treatment 5: Mineral

Researchers designed the mineral treatment to test Gaia F1’s response to a nutrient-rich substrate with enhanced macro- and micronutrients. Researchers increased EC and supplemented additional elements to stimulate metabolic activity, aiming to evaluate the effects on various parameters.

Treatment 6: Organic

This blend of slow-release organic nutrients mimicked living-soil or bio-amended systems. Organic regimes can influence the microbial population in the root zone, which in turn affects nutrient availability and secondary metabolite production. Testing Gaia F1 in this environment provided insight into how stable F1 cannabis seeds perform in lower-input, sustainability-oriented systems.

Rockwool Cannabis Growing: Stable Yields and Compact Plants

In rockwool conditions, Gaia F1 maintained exceptional consistency. Heights clustered tightly between 49 and 56 cm, demonstrating the variety’s stability. Yield per plant ranged from 116–133 g, with a mean area yield of about 0.80 kg/m² at 6 plants/m².

Control
Yield (g/plant) 133
Yield (kg/m²) 0.80
Height (cm) 56
Enhanced Nutrients
Yield (g/plant) 129
Yield (kg/m²) 0.78
Height (cm) 55
Reduced Nutrients
Yield (g/plant) 121
Yield (kg/m²) 0.75
Height (cm) 53
Rainwater
Yield (g/plant) 116
Yield (kg/m²) 0.70
Height (cm) 52
Low WC
Yield (g/plant) 118
Yield (kg/m²) 0.72
Height (cm) 49
Mineral
Yield (g/plant) 130
Yield (kg/m²) 0.79
Height (cm) 55
Organic
Yield (g/plant) 122
Yield (kg/m²) 0.75
Height (cm) 53

Research findings:

  • Rockwool produced a narrow variance in both height and yield.
  • Even stress treatments (rainwater, low WC) did not drastically disrupt morphology, confirming F1 hybrid stability.
  • The harvest index averaged 61%, with peaks at 69% under control conditions.

Coco Cannabis Growing: Higher Potential but Greater Variability

Coco cannabis growing showed Gaia F1’s capacity for higher single-plant yields but also revealed a greater spread between treatments. Plant height varied from 40 to 58 cm, and yields ranged from 57 to 136 g per plant. Average area yield reached 0.82 kg/m², slightly higher than rockwool’s but with larger swings.

Control
Yield (g/plant) 128
Yield (kg/m²) 0.77
Height (cm) 55
Enhanced Nutrients
Yield (g/plant) 132
Yield (kg/m²) 0.80
Height (cm) 56
Reduced Nutrients
Yield (g/plant) 105
Yield (kg/m²) 0.64
Height (cm) 50
Rainwater
Yield (g/plant) 57
Yield (kg/m²) 0.34
Height (cm) 40
Low WC
Yield (g/plant) 89
Yield (kg/m²) 0.54
Height (cm) 48
Mineral
Yield (g/plant) 136
Yield (kg/m²) 0.82
Height (cm) 58
Organic
Yield (g/plant) 118
Yield (kg/m²) 0.71
Height (cm) 52

Research findings:

  • Coco achieved the highest single-plant yield (mineral: 136 g/plant).
  • Rainwater and low WC treatments performed poorly, underscoring the importance of consistent irrigation.
  • Greater variability may appeal to advanced growers seeking to fine-tune conditions, but it’s riskier for scaled operations.

Comparative Insights: Rockwool vs Coco for Cannabis Yield and Stability

These cannabis substrate comparison results highlight the trade-offs between variables. Rockwool delivered predictable results, making it a potential preferred option for consistent production and demonstrating the stability of F1 hybrid cannabis seeds. In contrast, coco cannabis growing offered slightly higher potential yields but at the cost of stability.

The overall key patterns from this trial include:

  • Compact plants across both substrates confirm consistent morphology under varying conditions.
  • Poor performance with rainwater and WC suggests avoiding these treatments and prompts outdoor growers to develop holistic nutrient regimes.
  • Harvest index ranged from 54% to 69%, higher in rockwool than coco on average.
  • Under optimal conditions, pushing plant density could help growers achieve higher yields per area.
Comparison of Rockwool and Coco substrates, showing a plant rooted in green Rockwool and a circular Coco disc, tested for growth.

Choosing the Best Substrate for Cannabis F1 Hybrids (Rockwool vs Coco)

These Gaia F1 hybrid trial results provide a clear framework for selecting a substrate based on grower experience. For growers aiming at predictable yields with minimal risk, rockwool cannabis growing remains the top choice. Its tight performance band reflects strong F1 hybrid stability, making it easy to forecast output.

For more experienced growers seeking higher peaks, cultivating in coco can deliver, but only under carefully managed conditions. Your choice of substrate for cannabis F1 hybrids should depend on your skill level, facility controls, and tolerance for variability.

Practical tips:

  • Maintain consistent irrigation and nutrient regimes.
  • Avoid low water content or rainwater treatments for Gaia F1.
  • Increase plant density gradually to maximise the compact structure of Gaia F1.
  • Track EC and pH closely, especially in coco systems.

By aligning substrate choice with your operational goals, you can unlock the full potential of reliable F1 cannabis seeds, such as Gaia F1.

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Privacy Policy - Royal Queen Seeds

To ensure a safe online environment and guarantee adequate data protection, we strictly comply with all legal requirements. In this privacy statement, we provide information about how and for what purpose data is collected, safety measures, storage periods and contact details.


COMPANY NAME: SNORKEL SPAIN, SL (hereinafter ROYAL QUEEN SEEDS)

C/ Vilar d'Abdelà, 5 (nave 1) CP: 08170 de Montornès del Vallès

+34 937 379 846

[email protected]


The present Privacy Policy sets out the terms on which we will treat personal data at ROYAL QUEEN SEEDS; this includes any personal data collected through our website https://www.royalqueenseeds.com/ as well as any other data we process in the course of our business activities.

ROYAL QUEEN SEEDS collects the following personal data for the purposes listed below:

SECTION 1 – PERSONAL INFORMATION WE COLLECT

1.1 Account purchases

Account purchases can only be made if you are in possession of a personal account. When you create an account or purchase something from our shop, as part of the buying and selling process we collect the following personal information that you provide to us:

This information is required for delivery. In addition, when you browse our shop, we automatically receive the Internet Protocol (IP) address of your computer. Based on this information, we can optimise your online experience and at the same time protect our online environment.

Purpose of data collection

We collect and store account-related data for the following purposes:

(a) to carry out obligations arising from any contracts between you and us, and to provide you with information, products and services that you may request from us;

(b) to set up, manage and communicate with you about your account and your orders;

(c) to conduct market research and analysis;

(d) to confirm your age and identity, and to detect and prevent fraud.

1.2 Newsletters

With your explicit permission, we may send you newsletters about our shop, new products and other updates. We send newsletters based on your explicit consent. In the event that you purchase a product, and in accordance with current regulations, we may send you commercial communications in accordance with the legitimate interest of our company, always about products or services similar to those you have purchased or contracted. In any case, you may exercise your right of opposition through the channels announced in this Privacy Policy. The following information is collected in relation to the newsletter:

We do not need to know the sex of the person in order to send the newsletter (data minimisation: by law we must ask for data that is strictly necessary to provide the service, and in this case knowing the sex is not necessary to send the newsletter).

Purpose of data collection

The data collected is used to:

(a) personalise our emails, including your name and gender;

(b) provide gender-specific content.

You can withdraw your consent at any time by using the link provided in the newsletter or the contact information provided in section 2.

1.3 Customer service and contact form

In order to provide appropriate support, our customer service employees have access to information related to the account. Consequently, their support will be highly effective and friendly. The data provided in our contact form is used by our CRM provider, SuperOffice. We will only use your details to respond to your message.

SECTION 2 – LEGITIMATE INTEREST

If you have purchased any of our products, please note that we may process your personal data for promotional purposes, based on Royal Queen Seeds' legitimate interest only to offer you products or services from our company and about products or services similar to those you have purchased. You may exercise your right to opt out of future messages by the means set out in this Privacy Policy or through any notification you receive.

2.1 How do you withdraw consent?

If you change your mind, you can withdraw your consent for us to contact you for the purpose of collecting, using, or disclosing your data at any time by reaching out to us at: [email protected].

SECTION 3 – DISCLOSURE

We may disclose your personal information if we are required to do so by law or if you breach our Terms of Service.

SECTION 4 – HOW LONG DO WE KEEP YOUR DATA?

At Royal Queen Seeds, we will not retain your data for longer than is necessary for the purposes described in this Policy. Different retention periods apply for different types of data; however, the longest period we will normally hold any personal data is 10 years.

4.1 Account information

Data relating to the account remains relevant for as long as the consumer is in possession of an account. Therefore, the data remains documented for as long as the account exists. When our customers delete an account, the associated data will be deleted within a reasonable period of time. Requests regarding the inspection or correction of stored personal data or the deletion of an account can be sent to [email protected].

4.2 Newsletters

In the event that you give us your consent to inform you about our products or services, we will keep your data until you express your wish not to receive any further communications from us. However, we regularly (every month) carry out a relevance check. Registered customers (and their personal information) will be deleted whenever customers do not reply to our request. In addition, our newsletter mailing has an opt-out feature. Consumers can withdraw their consent by using this opt-out feature.

SECTION 5 – COOKIES

Cookies are small information files that notify your computer of previous interactions with our website. These cookies are stored on your hard drive, not on our website. Essentially, when you use our website, your computer displays its cookies to us, informing our site that you have visited before. This allows our website to function more quickly and remember aspects related to your previous visits (such as your username), making your experience more convenient. At Royal Queen Seeds, we use two types of cookies: functional and analytical.

5.1 Functional Cookies

Functional cookies are used to enhance your online experience. Among other things, these cookies track what is added to your shopping cart. The use of these cookies does not require prior authorization.

5.2 Analytical Cookies

Analytical cookies are used for research and market analysis. The data collected with these analytical cookies is anonymous, making it unusable for third parties. The use of these cookies does not require prior authorization.

SECTION 5 – THIRD-PARTY SERVICES

Third-party services are required to conduct transactions and provide our services. In general, the third-party providers we use will only collect, use and disclose your information to the extent necessary to enable them to perform the services they provide to us.

However, certain third-party service providers, such as payment gateways and other payment transaction processors, have their own privacy policies regarding the information we must provide to them for your transactions.

We encourage you to read the privacy policies of these providers so that you can understand how these providers will handle your personal information.

In particular, certain suppliers may be located or have facilities located in a different jurisdiction than yours or ours. Therefore, if you choose to proceed with a transaction involving the services of a third party, your information may be subject to the laws of the jurisdiction in which that service provider or its facilities are located.

Once you leave our website or are redirected to a third-party website or application, you are no longer governed by this Privacy Policy or the Terms of Service of our website.

Web analytics service (anonymous data)

On this website we have integrated an element of a web analytics service (with anonymisation functionality). Web analytics can be defined as the gathering, processing and analysis of data about the behaviour of visitors to websites. An analytics service collects, among other things, data about which website a person came from (the so-called referrer), which sub-pages they visited or how often and for how long they visited a sub-page. Web analytics is mainly used for website optimisation and for a cost–benefit analysis of internet advertising.

Courier service

To complete deliveries we use a courier service. This courier service carries out the delivery between our company and the consumer's home. To complete these logistics, the company requires access to the consumer's name and address information.

Mailing service

Royal Queen Seeds uses a third-party mail service provider to send its newsletter. This provider has access to limited account information related to opt-in consent (e.g. email address).

Marketing services

Royal Queen Seeds has the support of a company that specialises in marketing and communication activities. Their access to personal information is very limited and mostly anonymous.

Payment services

At Royal Queen Seeds we use external payment services to handle our transactions (e.g. credit card payments).

SECTION 6 – SECURITY

To protect your personal information, we take reasonable precautions and follow industry standard best practices to ensure that it is not inappropriately lost, misused, accessed, disclosed, altered or destroyed.

If you provide us with your credit card information, the information is encrypted using secure socket layer technology (SSL) and stored using AES-256 encryption. Although no method of transmission over the internet or electronic storage is 100% secure, we follow all PCI-DSS requirements and implement additional industry standards that are commonly accepted. Information related to the account is protected with a hashing method. This method transforms the information into a generated hash. As a result, confidential information is protected and invisible, even to us. In addition, our databases are exceptionally protected against unauthorised access. For example, access to the database is only possible and permitted via approved IP addresses (e.g. from Royal Queen Seeds headquarters). Other attempts and addresses are rejected at all times.

Furthermore, data is anonymised as much as possible, so it cannot be directly linked to a specific consumer. With this data, however, we may be able to carry out market research and analysis. In addition, the third parties concerned (e.g. mailing service) are examined prior to our collaboration, comply with the GDPR from the EU and receive a processing agreement. Within Royal Queen Seeds, employees are assigned different access permissions. Specific permission provides access only to information that is strictly required to perform a task. Digital security measures are subject to change and must meet high requirements to ensure the safety of online customers. That is why, at Royal Queen Seeds, we appoint a security officer. Regular verification and improvement of security measures (where necessary) are part of the role.

SECTION 7 – CHANGES TO THIS PRIVACY POLICY

We reserve the right to modify this Privacy Policy at any time, so please check back regularly. Changes and clarifications will be effective immediately upon being published on the Website. If we make substantial changes to this policy, we will notify you here that it has been updated so you will know what information we collect, how we use it and under what circumstances, if any, we use and/or disclose it.

SECTION 8 – YOU HAVE THE RIGHT TO: